Sap2蛋白与树突状细胞对系统性白假丝酵母菌感染的免疫保护性.pdfVIP

·1030· doi 10.3969/j.issn. 1673-4254.2014.07.23 J South Med Univ, 2014, 34(7): 1030-1034 基础研究 SapSap22蛋白与树突状细胞对系统性白假丝酵母菌感染的免疫保护蛋白与树突状细胞对系统性白假丝酵母菌感染的免疫保护性性 1 2 徐美兰 ，刘金星 1 2 嘉应学院医学院附属医院 检验科，中医科，广东 梅州 514031 摘要：目的小鼠进行重组蛋白与树突状细胞（DC）联合免疫，观察其对系统性白假丝酵母菌免疫保护效果。方法 原核表达并 诱导表达白假丝酵母菌Sap2蛋白；将白假丝酵母菌孢子、菌丝混悬液致敏小鼠DC后，分5组小鼠行3次免疫接种重组蛋白及致 敏DC、致敏DC、DC、Sap2、PBS，再造系统性白假丝酵母菌感染模型观察小鼠生存期、肾病理切片、肾负菌量、检测CD4+ 、CD8+ 百分率观察保护性。结果 成功构建pET32a-Sap2原核表达载体，目的蛋白被诱导表达并收集纯化。重组蛋白与致敏DC、DC、 Sap2蛋白均能有效延长系统性白假丝酵母菌感染小鼠生存期、保护小鼠肾脏、增强小鼠的免疫力，其中多组分联合免疫效果更 佳。结论 多组分联合免疫能很好地抵抗系统性白假丝酵母菌感染，这为研究新型多组分联合疫苗奠定基础。 关键词：天冬氨酸蛋白酶2；树突状细胞；系统性白假丝酵母菌感染；免疫保护 Immunoprotection by Sap2 and dendritic cells against systemic Candida albicans infection in mice 1 2 XU Meilan , LIU Jinxing 1Clinical Laboratory, 2Department of Traditional Chinese Medicine, Affiliated Hospital of Medical College, Jiaying University, Meizhou 514031, China Abstract: Objective To investigate the effect of combined immunization with recombinant Sap2 and dendritic cells (DCs) against systemic Canddida albicans infection. Methods We constructed a prokaryotic expression vector carrying sap2 of Candida albicans to obtain Sap2 protein. Murine DCs were sensitized by pulsing with Candida albicans spores and rope. Five groups of mice were immunized with recombinant Sap2 protein and sensitized DCs, sensitized DCs, naive DCs, Sap2, or PBS alone for 3 times, and the effect of the immunization against systemic Candida albicans infection were assessed by observing the survival of the mice, detecting the percentages of CD4+ and CD8+ cells, CFU in the kidney homogenate, and examining renal pathologies. Results Immunization with Sap2 and sensitized DCs and with DCs or Sap2 alone all prolonged the mouse survival and produced obvious effect in re