输尿管部分梗阻论文：肾盂尿蛋白和肾动态显象在评价肾积水肾损害程度中的实验研究.docVIP

输尿管部分梗阻论文：肾盂尿蛋白和肾动态显象在评价肾积水肾损害程度中的实验研究 【中文摘要】①探索建立一种较为理想的、可复性强的输尿管部分梗阻的动物模型。②研究肾盂尿N-乙酰-β-D-氨基葡萄糖苷酶（NAG）、视黄醇结合蛋白（RBP）、白蛋白（Alb）和肾动态显象在评价单侧输尿管部分梗阻所致肾损害程度评价中的临床意义③观察部分梗阻引起的肾积水、肾功能损害及肾纤维化的程度。方法：将50只雄性新西兰大白兔随机分组，假手术组（对照组）10只，只分离出左侧输尿管上段后直接关腹，手术入路选取背部肋弓下脊柱旁开2cm的纵行切口；手术组40只，分离出左侧输尿管后，将一根长约lcm内径1mm左右的聚乙烯塑料管纵形剖开，在肾盂输尿管连接部下约lcm处把输尿管套人其中，两端用1号丝线结扎固定。 【英文摘要】:①To establish an ideal and repeatable animal model of partialunilateral ureter obstruction(PUUO).②To evaluate the clinical significance of urinaryN-acetyl-β-D-glucosaminidase(NAG), Retinoid-binding-protein(RBP), Albumin(Alb)and renal dynamic imaging in degree of kidney damage caused by unilateral ureteralobstruction.③To investigate the degree of hydronephrosis, the change of renal function,and the development of tubulointerstitial fibrosis caused by PUUO.Methods：Fifty male New Zealand white rabbits were randomly allocated intosham operation group（control group）of 10 rabbits，only the left ureter was isolateddirectly before the abdomen was closed; Surgical approach chose a longitudinalincision on the back side under the costal arch about 2 cm away from the spine.The leftureters of 40 New Zealand white rabbits of surgery group were separated out ,thenplace a 1 mm diameter polyethylene plastic pipe around the ureter fixing the pipe bysilk thread. Surgery group was divided into A, B, C, D four groups randomly assigned10,and after surgery in 1, 2, 4, 8 weeks renal dynamic imaging were detected.Thencollect urine of left renal pelvis to test Alb, NAG, RBP, Cr. After eight weeks thecontrol group tested each index and get the renal dynamic imaging examination, theresults using the image analysis system for half quantitative analysis.Experimentalresults mean in x眘 , the comparisons among each group is using the single factoranalysis of variance by software SPSS 16.0, P 0.05 for with a statistical significance.After renal dynamic imaging examination and urine specimen collection, killed theanimals , Removing the damaged renals ,and ma