聚吡咯PLGA可导电复合纳米纤维支架对肝细胞生物相容性的研究.docxVIP

垦际生物医学工程杂志2014年lo月第37卷第5期IntJBiomedEng,October2014，V01．37，No．5·27l··论著·聚吡咯／PLGA可导电复合纳米纤维支架对肝细胞生物相容性的研究褚薛慧许茜冯章启肖江强李强孙喜太曹阳丁义涛【摘要】目的评估聚吡咯／PLGA可导电复合纳米纤维支架对肝细胞的生物相容性。方法采用静电纺丝及氧化结合法制备聚吡咯／PLGA可导电复合纳米纤维支架，并对其进行电镜表征及电化学检测。采用2步原位胶原酶法分离培养大鼠原代肝细胞。实验组将大鼠肝细胞培养于聚吡咯／PLGA可导电复合纳米纤维支架表面，而对照组设为PLGA培养组及空白对照组；采用MTI法检测各组细胞活性，BCA试剂盒评估各组细胞数量，ELISA法检测白蛋白分泌及尿素合成，同时还测定了各组肿瘤坏死因子一d(TNF一仅)及乳酸脱氢酶(LDH)的浓度。结果MTr实验及BCA检测提示聚吡咯／PLGA组与PLGA组间细胞数量及活性差异无统计学意义，但相比空白对照组其差异均具有统计学意义(PO．05o白蛋白分泌及尿素合成方面也有类似表现，聚吡咯肌GA组与PLGA组间差异无统计学意义，但较空白对照组要好。各组TNF—d及LDH的浓度差异无统计学意义(尸o．05)。结论聚吡咯／PLGA可导电复合纳米纤维支架具有良好的生物相容性，可以作为肝脏组织工程中肝细胞黏附介质的理想材料。【关键词】聚吡咯；肝细胞；纳米支架；生物相容性中图分类号：R318．08；Q813文献标识码：A文章编号：1673—4181(2014)05—0271—05BiocompatibiUtystudyofpolypyrrole／PLGAconductivenanofiberscaffoldonhepatocytesChuXuehui*，XuQin鸭屁ngZhangqi,XiaoJiangqiang,LiQi们岛SunXitai，CaoYang,DingYitao．+DepartmentofHepatobiliarySurgery,AffiliatedDrumTowerHospitalofMedicalSchool,NanjingUniversity,Nanjing210008,China【Abstract】ObjectiveToevaluatethebiocompatibilityofpolypyrrole／PLGAconductivenanofiberscaffoldonhepatocytes．MethodsThepolypyrrole／PLGAconductivenanofiberscaffoldwasdesignedviaelectrospinningandoxidativepolymerization，andcharacterizedbyscanningelectronmicroscope(SEM)andcyclicvohammetry．Rathepatocyteswereharvestedbyatwo—stepinsitucollagenaseperfusionprocedure．Ratsweredividedaspolypyrrole／PLGAgroup，PLGAgroupandtheblankcontrolgroup．ThecytoaetivityWasdetectedbyMTIassay，cellnumberwasassessedbyBCAkit，thealbuminsecretionandureasynthesisweredetectedbyenzyme-linkedimmunosorbentassay皿LISA)，andtheconcentrationoflactatedehydrogenase(LDH)andtumornecrosisfactor-or(TNF-a)wasalsomeasured．ResultsThereWasnosignificantdifferenceinthecellnumberandcytoactivitybetweenpolypyrrolefPLGAgroupandPLGAgroup(PO．05)，whichwerebothbetterthantheblankcontrolgroup(P0．05)．Theureasynthesisandalbuminsecretionofhepatoeytesonpolypyrrole／PLGAconductivescaffoldhadasimilarphenomenon．TherewasnosignificantdifferencebetweentheconcentrationofLDHandTNF-aamongthethreegroups(尸如．05)．ConclusionsThepolypyr