一年生簇毛麦醇溶蛋白基因的分离、原核表达与功能鉴定.pdf

作物学报 ACTA AGRONOMICA SINICA 2014, 40(8): 13401349 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2014.01340 一年生簇毛麦 α-醇溶蛋白基因的分离、原核表达与功能鉴定 杨 帆1 陈其皎1,2 高 翔1,2,* 赵万春1,2,* 强琴琴1 吴 丹1 孟 敏1 1 , 712100; 2 , 712100 : , α- 1 , 5 (Dasypyrum villosum) 52 , 816~873 bp (GenBank KJ004676~KJ004727) , 8 , 1 (KJ004680) , KJ004677KJ004686KJ004714 KJ004696 1 Cys, , 3 Tyr→Cys , KJ004696 Ser→Cys N-I , N α-5 Cys α- , KJ004708 (6 Cys)KJ004714 ( 1 Cys), IPTG 30 kD , ; , 2 α- , 4 g , KJ004708 KJ004714 , 1 Cys KJ004714 : ; α-; ; ; Cloning, Prokaryotic Expression and in vitro Functional Analysis of α-Gliadin Genes from Dasypyrum villosum 1 1,2 1,2,* 1,2,* 1 1 YANG Fan , CHEN Qi-Jiao , GAO Xiang , ZHAO Wan-Chun , JIANG Qin-Qin , WU Dan , and 1 MENG Min 1 College of Agronomy, Northwest AF University, Yangling 712100, China; 2 New Varieties Cultivation of Wheat Engineering Research Centre of Shaanxi Province, Yangling 712100, China Abstract: Gliadin, which has a great effect on wheat quality, is one of main components in gluten. According to the full lengths of α-gliadin genes deposited in NCBI database, a conserved primer pair was designed to clone α-gliadin genes in five Dasypyrum villosum lines. A total 52 sequences (816