一种烟粉虱成虫唾液酶鉴定与活性分析的方法-应用昆虫学报.pdfVIP

昆虫知识 Chinese Bulletin of Entomology 20 10 ，47 (5 ):10 17 ～ 1020 殤檻檻檻檻檻檻檻殤 檻 檻 檻 技术与方法檻 殤檻檻檻檻檻檻檻殤 一种烟粉虱成虫唾液酶鉴定与活性分析的方法* 1 ，2  2 2  1 彭 露 严 盈 万方浩 王进军 (1. 西南大学植物保护学院重庆市昆虫学及害虫控制工程重点实验室 重庆 4007 16 ; 2. ， 10008 1) 中国农业科学院植物保护研究所 植物病虫害生物学国家重点实验室 北京 1 ，2  2 A method to identify and analyze salivary enzymes of Bemisia tabaci adults. PENG Lu ，YAN Yin ， 2  1 WAN Fang-Hao ，WANG Jin-Jun (1. Key Laboratory of Entomology and Pest Control Eng ineering ，College of Plant Protection ，Southwest University ，Chongqing 4007 16 ，China ，2 . State Key Laboratory f or Biology of Plant Diseases and Insect Pests ，Institute of Plant Protection ，Chinese A cademy of Agricultural Sciences ，Beijing 10008 1 ，China) Abstract A method to identify and analyze the salivary enzymes of small piercing － sucking insects ，including artificial feeding ，saliva collection ，enzyme identification and analysis of salivary polyphenol oxidase and peroxidase ，was developed using Bemisia tabaci (Gennadius)B-biotype adults. The result show that PPO and POD activity in the watery saliva of B. tabaci B-biotype feeding on cabbage were respectively 1. 54 and 1. 65 fold higher than those feeding on tomato . This new method provides a simple intuitive and sensitive way of identifying and measuring the activity of enzymes in insect saliva. This method can also be applied to other small piercing － sucking insects ，such as aphids and psyllids. Key words piercing-sucking insect ，Bemisia tabaci ，saliva collectio