应用ISSR分子标志探讨台湾产香桂(樟科)之遗传变异.PDFVIP

29(1) 1-12, 2007 1 林業研究季刊 ： 研究報告 ISSR ( ) 1 應用 分子標誌探討台灣產香桂 樟科 之遺傳變異 何坤益2 (Cinnamomum subavenium Miq. (Lauraceae (Cinnamomum 【摘要】香桂 ）為樟科 ）樟屬 ）之重要 原生樹種，本研究應用簡單重複序列 (Inter-Simple Sequence Repeat, ISSR ）分子指紋技術，探討 9 72 17 ISSR 其族群的遺傳變異與親緣關係。分析臺灣自然分佈之 地區 個樣本，使用了 個 引子進 (Polymerase chain reaction PCR) 150 78 行聚合 鏈鎖反應 ； 得到 個條帶，其中多型性條帶有 條 (52%) 。分子變方分析(AMOVA) 結果，族群間之變方成分是16.45% (p ＜0.001) ，族群內的變方 83.55% (p 0.001) POPGENE 成分是 ＜ ，族群遺傳變異成分來自於族群內。 分析結果顯示，族群 Neis 基因歧異度值為0.3944 ，遺傳分化指數(Gst) 為0.3642 ，基因流(Nm) 為0.8730 。歸群分析 與主座標分析結果無地理親緣關聯，推測為生育環境之差異，族群基因流受限制，具有較高的基因 歧異度與遺傳分化現象。 【關鍵詞】遺傳變異、簡單重複序列、分子變方分析、香桂 Research paper Genetic Variation of Cinnamomum subavenium Miq. (Lauraceae) in Taiwan Using ISSR Markers1 Kuen-Yih Ho2 Abstract Cinnamomum subavenium Miq. is an endemically important species in Taiwan belonging 【 】 to the genus of Cinnamomum in Lauraceae family. In the present study as ISSR molecular markers and 17 corresponding primers were applied, 72 individuals collected from 9 sampling sites around Taiwan were studied and resulted in a total of 150 bands. Among them, 78 bands were polymorphic (52%) . Analysis of Molecular Variance (AMOVA) revealed that the component of variance among populations was 16.45% (p ＜0.001) and was attributed to the population differentiation. The main variance was coun