生物传感技术在食品农药残留检测中的应用.docVIP

生物传感技术在食品农药残留检测中的应用 满 燕, 梁 刚, 靳欣欣, 付海龙, 潘立刚农产品产地环境监测北京市重点实验室, 北京 100097) 摘 要: 农药的大量使用引发的食品安全问题日益突出。目前, 食品中农药残留的分析主要采用液相及液质联用、气相及气质联用等传统检测方法。这些方法具有较高检测灵敏性和选择性, 但却需要昂贵的仪器设备以及专业的技术人员, 不能满足当前食品安全领域对实时、快速、便携式检测的需求。生物传感技术作为农药检测的前景替代方法, 具有高专一性、高 关键词; 农药残留; 酶生物传感器; 免疫传感器; 检测 Application of biosensor techniques in pesticides detection in food MAN Yan, LIANG Gang, JIN Xin-Xin, FU Hai-Long, PAN Li-Gang* (Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, Risk Assessment Lab for agro-products (Beijing), Ministry of Agriculture, Beijing Research Center for Agricultural Standards and Testing,Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China) ABSTRACT: Food safety problems have become more and more serious due to the extensive use of pesticides. So far, the traditional detection methods, such as liquid chromatography (LC) and liquid chromatography-mass spectrometry (LC-MS), gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS), had been used for pesticides detection with the advantages of high sensitivity and selectivity. However, those methods required expensive equipments and highly qualified technicians. Moreover, it couldnt satisfy the needs of real-time, rapid and portable for the food safety areas. Biosensor techniques have become one of the most promising alternative methods for pesticides detection with the capabilities of high specificity, selectivity, portability and real-time analysis, which can make up the deficiency of the traditional detection methods. The enzyme/antibody-based biosensors for pesticide detection were reviewed firstly in this paper. Secondly, the new biosensor technologies based on aptamer, microfluidic chip and molecularly imprinted polymer (NIP) were introduced in detail as well. Finally, the development prospects of the biosensors for the pesticides detection were previewed. KEY WORDS: food; pesticides; enzyme biosensor; immunosensor; detection 1 引 言 “民以食为天, 食以安为先”, 食品是人类赖以生