波纹龙虾c-型凝集素phleca的基因克隆与表达-pergikejtoaccn.pdfVIP

热带海洋学报 JOURNAL OF TROPICAL OCEANOGRAPHY 2016 年 第 35 卷 第 5 期: 29 海洋生物学 doi:10.11978/2015155 C-PhLecA * 李斌, 梁华芳, 陈兆明, 黄启幸, 程志芬, 陈仕坤 , 524088 : C-Ca2+, , RT-PCR (rapid amplification of cDNA ends, RACE) Panulirus homaus C-PhLecA cDNA , 863bp, 729 bp, 243 , 17 CRD(carbohydrate recognition domain) RT-PCR , PhLecA mRNA , , , PCR WSSV(white spot syndrome virus) PhLecA , , WSSV, PhLecA , , , PhLecA : ; C-; ; ; 中图分类号: P735.542 文献标识码: A 文章编号: 1009-5470(2016)05-0029-09 Gene cloning and analysis of a novel C-type lectin PhLecA from Panulirus homaus LI Bin, LIANG Huafang, CHAN Siuming, HUANG Qixing, CHENG Zhifen, CHEN Shikun Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China Abstract: C-type lectins (CTLs) are a large family of proteins that exist in all deuterostomia. CTLs can bind to carbohydrate moieties normally in a calcium-dependent manner and play important roles in immune defense. The cDNA sequence of Panulirus homaus LecA (PhLecA) was obtained using rapid amplification of cDNA ends method (RACE). The full-length cDNA sequence of PhLecA was 863 bp, which contained a 729 bp complete open reading frame (ORF) encoding 243 amino acids. The deduced amino acid sequence of PhLecA had a signal peptide containing 17 amino acid residues and a carbohydrate recognition domain (CRD). RT-PCR analysis showed that the mRNA of PhLecA was widely distributed in various tissues in the embryonic period and the larval stage, with highest expression in hepatopancreas and then in stomach, intestine a