通过DNA芯片识别目标基因技术提高棒状杆菌赖氨酸生产能力.pdf

Appl Microbiol Biotechnol (2007) 76:677–689 DOI 10.1007/s00253-007-0916-x GENOMICS AND PROTEOMICS Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes Georg Sindelar Volker F. Wendisch Received: 19 January 2007 /Revised: 27 February 2007 /Accepted: 27 February 2007 / Published online: 16 March 2007 # Springer-Verlag 2007 Abstract For the biotechnological production of L-lysine, lysine production are present. NCgl0855, putatively encod- mainly strains of Corynebacterium glutamicum are used, ing a methyltransferase, and the amtA-ocd-soxA operon, which have been obtained by classical mutagenesis and encoding an ammonium uptake system, a putative ornithine screening or selection or by metabolic engineering. Gene cyclodeaminase and an uncharacterized enzyme, were targets for the amplification and deregulation of the among the genes showing increased expression in the lysine biosynthesis pathway, for the improvement of classically obtained strain irrespective of the presence of carbon precursor supply and of nicotinamide adenine feedback-resistant aspartokinase. Lysine production could dinucleotide phosphate (reduced form) (NADPH) regen- be improved by about 40% through overexpression of eration, are known. To identify novel target genes to NCgl0855 or the amtA-ocd-soxA operon. Thus, novel target improve lysine production, the transcriptomes of the genes for the improvement of lysine production could be classically obtained lysine producing strain MH20-22B identified in a discovery-driven approach based on global and several other C. glutamicum strains were compared.