论文：缩醛磷脂酰乙醇胺对羟自由基所致大鼠脑线粒体氧化损伤的保护作用.docVIP

缩醛磷脂酰乙醇胺对羟自由基所致大鼠脑线粒体氧化损伤的保护作用 张树平1，田京伟2 ，杨建雄3 ，王垣芳1 1 山东省滨州医学院药理学教研室（烟台校区），山东 烟台 264003 2 山东省烟台大学药学院，山东省天然药物工程技术研究中心 山东 烟台 264003 3 陕西师范大学生命科学学院 陕西 西安 710062 摘要：目的 研究缩醛磷脂酰乙醇胺对羟自由基所致大鼠脑线粒体损伤的保护作用。方法 利用Fe2++VitC系统产生· OH，诱导大鼠脑线粒体损伤；测定线粒体肿胀度、膜流动性、膜磷脂含量、呼吸功能、线粒体呼吸酶、超氧化物歧化酶（SOD）、丙二醛（MDA）、Ca2+ 等指标以显示线粒体膜功能、呼吸功能、抗氧化能力的变化。结果 ·OH 造成线粒体显著损伤，缩醛磷脂酰乙醇胺（终浓度0.03、0.06、0.12、0.24、0.48 mmol·L-1）明显抑制膜磷脂降解、线粒体肿胀，增加膜流动性，改善线粒体能量代谢，增强抗氧化能力。结论 缩醛磷脂酰乙醇胺对·OH所致大鼠脑线粒体损伤有明显保护作用，其机制与减少自由基对线粒体膜的损伤、抑制脂质过氧化有关。 关键词 缩醛磷脂酰乙醇胺；线粒体；羟自由基；能量代谢 Protective effect of plasmenylethanolamine against rat cortex mitochondria injury induced by 稯H ZHANG Shu-ping1, 护TIAN Jing-wei 2, YANG Jian-xiong3,WANG Yuan-fang1 (1.Department of Pharmacology, Binzhou Medical College, Yantai Shandong 264003, China; 2.School of Pharmaceutical Science, Shandong Yantai University; Shandong Engineering Research Center for Natural Drugs, Yantai Shandong 264003, China; 3.College of Life Science, Shaanxi Normal University, Xi’an Shaanxi 710062, China) ABSTRACT:OBJECTIVE To study the effects of plasmenylethanolamine on cortex mitochondria injuries induced by ·OH in rats. METHODS Fe2++VitC system to produce ·OH were available, cortex mitochondria of rats were isolated, membrane fluidity, swelling, respiratory function, activities of mitochondria respiratory enzymes, contents of phospholipid and Ca2+ were determined to evaluate the ability of mitochondria energy metabolism. The activity of superoxide dismutase (SOD) and contents of malondialdehyde (MDA) were also determined to evaluate the antioxygenation ability of mitochondria. RESULTS The data indicated that ·OH resulted in severe neuronal mitochondria injuries, Which could be alleviated by plasmenylethanolamine (content of 0.12，0.24 and 0.48 mmol·L-1). The swelling of mitochondria was ameliorated, the decomposability of mitochondria membrane phospholipid was decreased, membrane fluidity of mitochondria was increased, plasmenylethanolamine also significantly inhibited the decrease in