血卟啉衍生物介导光动力疗法对人肺腺癌细胞杀伤-第三军医大学学报.docVIP

血卟啉衍生物人肺癌细胞杀伤的 研究血卟啉衍生物（hematoporphrphyrin derivative, HpD）介导的光动力疗法（photodynamic therapy, PDT）对在体和离体人肺腺癌细胞A549的杀伤作用。方法应用流式细胞术分析对细胞的影响。549细胞裸鼠移植瘤动物模型，绘制肿瘤生长曲线观察PDT对肺癌生长抑制作用，TUNEL法检测肿瘤细胞凋亡。结果 HpDPDT作用后，细胞显著高于对照组（P0.05）抑制效率依赖HpD浓度及激光剂量裸鼠移植瘤动物模型（P0.01）。结论 HpDPDT可有效杀伤肺腺癌细胞，其作用机制可能与抑制肿瘤细胞增殖诱导细胞凋亡有关。血卟啉衍生物肺癌mpirical study on lethal effect of hematoporphyrin derivative mediated photodynamic therapy on human adenocarcinoma of lung cells YANG Zhen-zhou, ZHANG Yun-song, LI Meng-xia, ZENG Lin-li, LI Zeng-peng, WANG Dong, WANG Ge （Center of Oncology, Daping Hospital and Research Institute of Surgery, the Third Military Medical University, Chongqing 400042, China） Abstract: Objective To investigate lethal effect of hematoporphrphyrin derivative (HpD) mediated photodynamic therapy (PDT) on human adenocarcinoma of lung cells A549 in vivo and in vitro. To provide theoretical evidence for treating lung cancer by HpD-PDT. Methods Dose-effect relationship of HpD-PDT were detected on proliferative inhibition of A549 cells by MTT. Flow cytometry was used to analyses apoptosis. Furthermore, to construct A549 xenograft in nude mice, HpD-PDT inhibited the growth of lung cancer cells to be observed by growth curve experiment, and apoptosis was detected by TUNEL. Results After HpD-PDT treated, proliferative inhibition and apoptosis of A549 cells were significant more than control group (P0.05), and they depended on HpD density and laser dose. Growth inhibition of tumor and apoptosis of HpD-PDT group had also significant difference (P0.05) than control group in A549 xenograft in nude mice. Conclusion HpD-PDT can kill effectively adenocarcinoma cells of lung. It is possible mechanism that HpD-PDT can inhibit proliferation of tumor cell and induce apoptosis. Key words: hematoporphrphyrin derivative; photodynamic therapy; APE1; adenocarcinoma of lung 基金项目：国家自然科学基金 Supported by the National Natural Science Foundation of China 作者简介：杨镇洲，男，浙江省浦江县人，副主任医师，副教授，主要从事肿瘤放射性损伤与修复方面的研究，发