中西医联合用药对糖尿病大鼠视网膜神经节细胞bcl2及bax基因表.docVIP

中西医联合用药对糖尿病大鼠视网膜神经节细胞bcl2及bax基因表 　【摘要】 [目的]观察不同治疗方法对糖尿病大鼠视网膜神经节细胞bcl2和bax基因表达的影响。[方法]将40只雄性SD大鼠随机分成正常对照组、糖尿病对照组、盐酸二甲双胍治疗组、中药治疗组和中西医联合用药组，小剂量两次腹腔注射链脲佐菌素诱发糖尿病大鼠模型。中药治疗组和中西医联合用药组在造模前15天就进行预防性给药(以中药补肾通窍方煎剂灌胃)。西药组和中西医联合用药组在成模后给以盐酸二甲双胍给药。成模6W后将实验大鼠脱臼处死，取出眼球加入液氮磨碎，提取RNA，进行PCR电泳，再进行荧光PCR扩增，检测视网膜神经节细胞bcl2和bax基因表达的情况。[结果]中西医联合用药组大鼠视网膜神经节细胞bcl2和bax表达跟其它组比较具有显著性差异(Plt;0.05)，有统计学意义。[结论]中西医联合用药可以有效影响糖尿病大鼠视网膜神经节细胞bcl2和bax基因的表达，从而抑制糖尿病大鼠视网膜神经节细胞的凋亡。 【关键词】 糖尿病大鼠;神经节细胞;基因;中西医 　　Abstract：[Objective]To observe the effect of different treatments on bcl2 and bax genetic expression of retinal ganglion cell in rats with diabetic retinopathy.[Methods]Forty male SD rats were randomly divided into 5 groups： normal group(N group )，diabetic group(D group)，metformin hydrochiloride treated group(MH group)，Chinese medicine treated group(C group)，Chinese western medicine combined treated group(CW group).D group was set up by injecting STZ twice in small volumes.In the C and CW groups，rats were orally administered with Bushentongqiao Formulation liquid for 15 days.Metformin hydrochloride was orally administered into the rats in MH and CW groups.Six weeks later，all the rats were killed and their eye balls were taken out and pulverized with fluid nitrogen to powder，from which RNA was extracted.Then RNA was electrophoresized and expanded in the way of PCR and fluorescent quantitative PCR respectively so as to investigate the bcl2 and bax genetic expression of retinal ganglion cell in rats with diabetic retinopathy.[Result]The bcl2 and bax genetic expression of retinal ganglion cell in the rats of CW group is significantly different from that in other groups，showing the statistical significance.[Conclusion]The Chinese western combined treatment has significant effect on bcl2 and bax genetic expression of retinal ganglion cell in rats with diabetic retinopathy,With a result of suppressing the apoptosis of retinal cell in diabetic rats. 　　Key words： diabetic rats;retinal cell;gene;Chinese and we