IL18基因转染对小鼠卵巢癌OVHM细胞体内成瘤影响.doc

IL18基因转染对小鼠卵巢癌OVHM细胞体内成瘤影响 　 作者：崔澄 郝淑维 白小嘉 李新伟 周强 程建新 单保恩 【摘要】 目的 分析转染IL18基因后，对小鼠卵巢癌OVHM细胞体内成瘤的影响，初步探讨IL18基因转染治疗卵巢癌的应用价值。方法 将逆转录病毒携带的小鼠IL18基因成功转染至OVHM（OVHM/IL18），以空载体转染的OVHM（OVHM/LXSN）和野生型（OVHM）作为对照，于裸鼠皮下分别接种细胞，观察各组种植瘤生长情况，计算成瘤率；HE染色及电镜分别观察病理组织切片的形态学改变。结果 三组裸鼠的成瘤率相同，但OVHM/IL18组成瘤时间较晚，随瘤龄增长肿瘤生长缓慢；瘤组织较小、界限清楚，血供不丰富，瘤细胞数量少、恶性程度较低，可见中性粒细胞、淋巴细胞浸润及细胞凋亡、组织坏死现象。OVHM组和OVHM/LXSN组的瘤组织较大、与周围组织界限不清，血管丰富，瘤细胞密集、恶性程度较高。结论 IL18基因成功转染后，可能通过促进肿瘤细胞凋亡、抑制肿瘤血管形成及促进免疫浸润等机制，达到抑制肿瘤生长、降低卵巢癌恶性程度的抗瘤作用。 【关键词】 IL18；基因转染；OVHM；成瘤；裸鼠 　　【Abstract】 Objective To investigate the effect of IL18 gene transfection on tumorigenesis of mouse ovarian cancer cell line OVHM, and to discuss the preliminary value of IL18 gene transfection in treatment of ovarian cancer. Methods OVHM was transfected with retrovirus with IL18 gene named as OVHM/IL18, and the wild OVHM and OVHM transfected with vector without IL18 gene (OVHM/LXSN) were enrolled as control. The nude mice were inoculated with different cells subcutaneously, then the growth of transplanted tumor was observed, the ratios of tumorigenesis were calculated and the morphology of tumor cell in tissue was analyzed by HE staining and electron microscope. Results After inoculated respectively, the ratios of tumorigenesis were similar in three mice groups. But in OVHM/IL18 group, the latent period of tumorigenesis was longer than that of other two groups, the slower speed along with tumor growth was observed; the tumor tissue was smaller and had a limited boundary, the number of blood vessel and tumor cells were fewer, and the malignance of tumor cells was lower;the apoptosis, coagulative necrosis and the infiltration of neutrophilic granulocytes and lymphocytes were observed. Meanwhile, the typical morphology of apoptosis was showed by electron microscope. However, the tumor tissues of OVHM and OVHM/LXSN groups were larger and had unclear boundary, the number of blood vessel and tumor cells were more, the malignance of tumor cells was higher. Con