detection of head-to-tail dna sequences of human bocavirus in clinical samples检测头部到尾部人类bocavirus临床样本的dna序列.pdfVIP

Detection of Head-to-Tail DNA Sequences of Human Bocavirus in Clinical Samples ¨ 1. 1. 1 1¤a ¨ 1¤b Jessica Lusebrink , Verena Schildgen , Ramona Liza Tillmann , Felix Wittleben , Anne Bohmer , ¨ 2 1 Andreas Muller , Oliver Schildgen * ¨ ¨ ¨ ¨ 1 Institut fur Pathologie, Kliniken der Stadt Koln gGmbH, Klinikum der Privaten Universitat Witten-Herdecke, Koln (Cologne), Germany, 2 Department of Paediatrics, University Hospital Bonn, Bonn, Germany Abstract Parvoviruses are single stranded DNA viruses that replicate in a so called ‘‘rolling-hairpin’’ mechanism, a variant of the rolling circle replication known for bacteriophages like QX174. The replication intermediates of parvoviruses thus are concatemers of head-to-head or tail-to-tail structure. Surprisingly, in case of the novel human bocavirus, neither head-to-head nor tail-to- tail DNA sequences were detected in clinical isolates; in contrast head-to-tail DNA sequences were identified by PCR and sequencing. Thereby, the head-to-tail sequences were linked by a novel sequence of 54 bp of which 20 bp also occur as conserved structures of the palindromic ends of parvovirus MVC which in turn is a close relative to human bocavirus. ¨ ¨ Citation: Lusebrink J, Schildgen V, Tillmann RL, Wittleben F, Bohmer A, et al. (2011) Detection of Head-to-Tail DNA Sequences of Human Bocavirus in Clinical Samples. PLoS ONE 6(5): e19457. doi:10.1371/journal.pone.0019457 Editor: Volker Thiel, K