designer lipid-like peptides a class of detergents for studying functional olfactory receptors using commercial cell-free systems而类脂多肽的洗涤剂类研究颗粒系统的功能使用商业嗅觉受体.pdfVIP

Designer Lipid-Like Peptides: A Class of Detergents for Studying Functional Olfactory Receptors Using Commercial Cell-Free Systems 1 2 1 1 1 1,4 Karolina Corin , Philipp Baaske , Deepali B. Ravel , Junyao Song , Emily Brown , Xiaoqiang Wang , 3 3 2 5 3 Christoph J. Wienken , Moran Jerabek-Willemsen , Stefan Duhr , Yuan Luo , Dieter Braun , Shuguang Zhang1* 1 Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, 2 NanoTemper Technologies GmbH, ¨ ¨ Munchen, Germany, 3 Systems Biophysics, Functional Nanosystems, Ludwig-Maximilians University Munich, Munchen, Germany, 4 Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong, China, 5 Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, Maryland, United States of America Abstract A crucial bottleneck in membrane protein studies, particularly G-protein coupled receptors, is the notorious difficulty of finding an optimal detergent that can solubilize them and maintain their stability and function. Here we report rapid production of 12 unique mammalian olfactory receptors using short designer lipid-like peptides as detergents. The peptides were able to solubilize and stabilize each receptor. Circular dichroism showed that the purified olfactory receptors had alpha-helical secondary