decrease in pneumococcal co-colonization following vaccination with the seven-valent pneumococcal conjugate vaccine减少肺炎球菌疫苗接种后co-colonization seven-valent肺炎球菌结合疫苗.pdfVIP

Decrease in Pneumococcal Co-Colonization following Vaccination with the Seven-Valent Pneumococcal Conjugate Vaccine Carina Valente1,2, Jason Hinds3, Francisco Pinto4, Silvio D. Brugger5, Katherine Gould3, Kathrin ¨ 5 ´ 2,6 ´ ˜ 1,2 Muhlemann , Hermı nia de Lencastre , Raquel Sa-Leao * ´ ´ 1 Laboratory of Molecular Microbiology of Human Pathogens, Instituto de Tecnologia Quımica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal, 2 Laboratory of ´ ´ Molecular Genetics, Instituto de Tecnologia Quımica e Biologica, Universidade Nova de Lisboa, Oeiras, Portugal, 3 Bacterial Microarray Group, St. George’s, University of ´ ´ ´ ´ ˆ London, London, United Kingdom, 4 Centro de Quımica e Bioquımica, Departamento de Quımica e Bioquımica, Faculdade de Ciencias da Universidade de Lisboa, Lisbon, Portugal, 5 Institute for Infectious Diseases, University of Bern, Bern, Switzerland, 6 Laboratory of Microbiology, The Rockefeller University, New York, New York, United States of America Abstract Understanding the epidemiology of pneumococcal co-colonization is important for monitoring vaccine effectiveness and the occurrence of horizontal gene transfer between pneumococcal strains. In this study we aimed to evaluate the impact of the seven-valent pneumococcal conjugate vaccine (PCV7) on pneumococcal co-colonization among Portuguese children. Nasopharyngeal samples from children up to 6 years old yielding a pneumococcal culture were clustered into three groups: pre-vaccine era (n = 173), unvaccinated children