deacetylation of chitosan material characterization and in？vitro evaluation via albumin adsorption and pre-osteoblastic cell cultures壳聚糖的脱乙酰作用材料表征和体外评价通过白蛋白吸附和pre-osteoblastic细胞培养.pdfVIP

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deacetylation of chitosan material characterization and in？vitro evaluation via albumin adsorption and pre-osteoblastic cell cultures壳聚糖的脱乙酰作用材料表征和体外评价通过白蛋白吸附和pre-osteoblastic细胞培养.pdf

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deacetylation of chitosan material characterization and in？vitro evaluation via albumin adsorption and pre-osteoblastic cell cultures壳聚糖的脱乙酰作用材料表征和体外评价通过白蛋白吸附和pre-osteoblastic细胞培养

Materials 2011, 4, 1399-1416; doi:10.3390/ma4081399 OPEN ACCESS materials ISSN 1996-1944 /journal/materials Article Deacetylation of Chitosan: Material Characterization and in vitro Evaluation via Albumin Adsorption and Pre-Osteoblastic Cell Cultures Youling Yuan *, Betsy M. Chesnutt, Warren O. Haggard and Joel D. Bumgardner Department of Biomedical Engineering, University of Memphis, Memphis, TN 38152, USA; E-Mails: betsy.chesnutt@ (B.M.C.); whaggrd1@ (W.O.H.); jbmgrdnr@ (J.D.B.) * Author to whom correspondence should be addressed; E-Mail: ylyuan@; Tel.: +1-502-852-0825.; Fax: +1-502-852-2123. Received: 21 July 2011 / Accepted: 5 August 2011 / Published: 12 August 2011 Abstract: Degree of deacetylation (DDA) and molecular weight (MW) of chitosans are important to their physical and biological properties. In this study, two chitosans, HS (DDA = 73.3%) and AT (DDA = 76.8%), were deacetylated with 45% sodium hydroxide under nitrogen atmosphere at 80 °C or 90 °C for up to 120 min, to obtain two series of chitosans. The polymers produced were characterized for MW by gel permeation chromatography, DDA by titration and UV-vis methods, and crystallinity, hydrophilicity and thermal stability by X-ray di raction, water contact angle and di erential scanning calorimetry respectively. Films, made by solution casting in