complete genome and transcriptomes of streptococcus parasanguinis fw213 phylogenic relations and potential virulence mechanisms完整的基因组和转录组链球菌parasanguinis fw213系统发生的关系和潜在的毒性机制.pdfVIP

Complete Genome and Transcriptomes of Streptococcus parasanguinis FW213: Phylogenic Relations and Potential Virulence Mechanisms 1 2,3 3,4 1,5 3,6 1 Jianing Geng , Cheng-Hsun Chiu , Petrus Tang , Yaping Chen , Hui-Ru Shieh , Songnian Hu , Yi- Ywan M. Chen3,6* 1 Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, People’s Republic of China, 2 Division of Pediatric Infectious Diseases, Molecular Infectious Disease Research Center, Chang Gung Children’s Hospital, Tao-Yuan, Taiwan, 3 Graduate Institute of Basic Medical Sciences, Chang Gung University, Tao-Yuan, Taiwan, 4 Bioinformatics Center, Chang Gung University, Tao-Yuan, Taiwan, 5 Graduate University of the Chinese Academy of Sciences, Beijing, People’s Republic of China, 6 Department of Microbiology and Immunology, Chang Gung University, Tao-Yuan, Taiwan Abstract Streptococcus parasanguinis, a primary colonizer of the tooth surface, is also an opportunistic pathogen for subacute endocarditis. The complete genome of strain FW213 was determined using the traditional shotgun sequencing approach and further refined by the transcriptomes of cells in early exponential and early stationary growth phases in this study. The transcriptomes also discovered 10 transcripts encoding known hypothetical proteins, one pseudogene, five transcripts matched to the Rfam and additional 87 putative small RNAs within the intergenic regions defined by the GLIMMER analysis. The genome contains five acquired genomic islands (GIs) encoding proteins which potentially contribute to the overall pathogenic capacity and fitness of this microbe. The