comparative transcript profiling of a male sterile cybrid pummelo and its fertile type revealed altered gene expression related to flower development比较分析记录的雄性不育胞质杂种柚子和肥沃的类型显示花的发育相关基因表达改变.pdfVIP

Comparative Transcript Profiling of a Male Sterile Cybrid Pummelo and Its Fertile Type Revealed Altered Gene Expression Related to Flower Development 1 1 1 1 2 1 Bei-Bei Zheng , Xiao-Meng Wu , Xiao-Xia Ge , Xiu-Xin Deng , Jude W. Grosser , Wen-Wu Guo * 1 Key Laboratory of Horticultural Plant Biology (Ministry of Education); National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, People’s Republic of China, 2 Citrus Research and Education Center, University of Florida, Lake Alfred, Florida, United States of America Abstract Male sterile and seedless characters are highly desired for citrus cultivar improvement. In our breeding program, a male sterile cybrid pummelo, which could be considered as a variant of male fertile pummelo, was produced by protoplast fusion. Herein, ecotopic stamen primordia initiation and development were detected in this male sterile cybrid pummelo. Histological studies revealed that the cybrid showed reduced petal development in size and width, and retarded stamen primordia development. Additionally, disorganized cell proliferation was also detected in stamen-like structures (fused to petals and/or carpel). To gain new insight into the underlying mechanism, we compared, by RNA-Seq analysis, the nuclear gene expression profiles of floral buds of the cybrid with that of fertile pummelo. Gene expression profiles which identified a large number of differentially expressed genes (DEGs) between the two lines were captured at both petal primordia and stamen primordia distinguishable stages. For example, nuclear genes involved in nucleic acid binding and response to hormone synthesis and metabolism, genes required for floral bud identification and expr