comparative proteomic analysis of streptococcus suis biofilms and planktonic cells that identified biofilm infection-related immunogenic proteins比较蛋白质组学分析的链球菌生物膜和浮游细胞识别生物膜感染相关性免疫原性的蛋白质.pdfVIP

Comparative Proteomic Analysis of Streptococcus suis Biofilms and Planktonic Cells That Identified Biofilm Infection-Related Immunogenic Proteins 1,2 1 1 1 1 1 1 1 Yang Wang , Li Yi , Zongfu Wu , Jing Shao , Guangjin Liu , Hongjie Fan , Wei Zhang *, Chengping Lu * 1 Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, China, 2 College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China Abstract Streptococcus suis (SS) is a zoonotic pathogen that causes severe disease symptoms in pigs and humans. Biofilms of SS bind to extracellular matrix proteins in both endothelial and epithelial cells and cause persistent infections. In this study, the differences in the protein expression profiles of SS grown either as planktonic cells or biofilms were identified using comparative proteomic analysis. The results revealed the existence of 13 proteins of varying amounts, among which six were upregulated and seven were downregulated in the Streptococcus biofilm compared with the planktonic controls. The convalescent serum from mini-pig, challenged with SS, was applied in a Western blot assay to visualize all proteins from the biofilm that were grown in vitro and separated by two-dimensional gel electrophoresis. A total of 10 immunoreactive protein spots corresponding to nine unique proteins were identified by MALDI-TOF/TOF-MS. Of these nine proteins, five (Manganese-dependent superoxide dismutase, UDP-N-acetylglucosamine 1-carboxyvinyltransferase, ornithine carbamoyl- transferase, phosphoglycerate kinase, Hypothetical protein SSU05_0403) had no previously reported immunogenic