binding of human milk to pathogen receptor dc-sign varies with bile salt-stimulated lipase (bssl) gene polymorphism绑定的母乳病原体受体dc-sign随胆汁salt-stimulated脂肪酶(bssl)基因多态性.pdfVIP

Binding of Human Milk to Pathogen Receptor DC-SIGN Varies with Bile Salt-Stimulated Lipase (BSSL) Gene Polymorphism 1 1 2 3 3 Martijn J. Stax , Marloes A. Naarding , Michael W. T. Tanck , Susanne Lindquist , Olle Hernell , Robert 4 5 6 1 1 Lyle , Per Brandtzaeg , Merete Eggesbø , Georgios Pollakis , William A. Paxton * 1 Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam (CINIMA), Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands, 2 Department of Clinical Epidemiology, Biostatistics and Bioinformatics, Academic Medical Center, University of ˚ ˚ Amsterdam, Amsterdam, The Netherlands, 3 Pediatrics Unit, Department of Clinical Sciences, Umea University, Umea, Sweden, 4 Department of Medical Genetics, Oslo University Hospital, Oslo, Norway, 5 LIIPAT, Centre for Immune Regulation, University of Oslo, and Department of Pathology, Oslo University Hospital, Rikshospitalet, Oslo, Norway, 6 Division of Epidemiology, Department of Genes and Environment, Norwegian Institute of Public Health, Oslo, Norway Abstract Objective: Dendritic cells bind an array of antigens and DC-SIGN has been postulated to act as a receptor for mucosal pathogen transmission. Bile salt-stimulated lipase (BSSL) from human milk potently binds DC-SIGN and blocks DC-SIGN mediated trans-infection of CD4+ T-lymphocytes with HIV-1. Objective was to study variation in DC-SIGN binding properties and the relation between DC-SIGN binding capacity of milk and BSS