β-defensin 2 as an adjuvant promotes anti-melanoma immune responses and inhibits the growth of implanted murine melanoma in vivoβ-defensin 2作为一个辅助促进anti-melanoma免疫反应,抑制体内植入小鼠黑色素瘤的生长.pdfVIP

b-defensin 2 as an Adjuvant Promotes Anti-Melanoma Immune Responses and Inhibits the Growth of Implanted Murine Melanoma In Vivo 1,2,3,4. 1,2. 1 1 1,2,4 1,4 Han-fang Mei , Xiao-bao Jin , Jia-yong Zhu *, Ai-hua Zeng , Qiang Wu , Xue-mei Lu , Xiao-bo Li1,2,4, Juan Shen1,4 1 Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, China, 2 School of Basic Sciences, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, China, 3 Department of Biochemistry and Molecular Biology, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, China, 4 Institute of Tropical Medicine and Public Health, Southern Medical University, Guangzhou, China Abstract b-defensin 2 is a small antimicrobial peptide of the innate immune system and has been thought to regulate anti-tumor immunity. However, little is known on whether b-defensin 2 could modulate melanoma-specific NK and T cell responses. In this study, we first cloned the murine b-defensin 2 gene by RT-PCR and generated the b-defensin 2 stably expressing B16 cells (B16-mBD2). Subsequently, we evaluated whether vaccination with irradiated B16-mBD2 could modulate the growth of implanted B16 cells and determined the potential mechanisms underlying the action of B16-mBD2 vaccine in modulating the growth of B16 tumors in C57BL/6. We found that vaccination with irradiated B16-mBD2, but not with control B16-p or parental B16, inhibited the development and progression of B16 tumors, and prolonged the survival of tumor-bearing mice. However, vaccination with irradiated B16-mBD2 fail