uniform silica coated fluorescent nanoparticles synthetic method, improved light stability and application to visualize lymph network tracer均匀涂二氧化硅荧光纳米粒子的合成方法,改进的光稳定性和应用可视化网络淋巴示踪剂.pdfVIP

Uniform Silica Coated Fluorescent Nanoparticles: Synthetic Method, Improved Light Stability and Application to Visualize Lymph Network Tracer 1 1 2 1 3 Liman Cong , Motohiro Takeda *, Yohei Hamanaka , Kohsuke Gonda , Mika Watanabe , Masutaka 4 5 4 1,2 Kumasaka , Yoshio Kobayashi , Masaki Kobayashi , Noriaki Ohuchi 1 Department of Nano-Medical Science, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan, 2 Department of Surgical Oncology, Graduate School of Medicine, Tohoku University, Sendai, Miyagi, Japan, 3 Department of Pathology, Tohoku University Hospital, Sendai, Miyagi, Japan, 4 Department of Electronics and Intelligent Systems, Tohoku Institute of Technology, Sendai, Miyagi, Japan, 5 Department of Biomolecular Functional Engineering, College of Engineering, Ibaraki University, Hitachi, Ibaraki, Japan Abstract Background: The sentinel lymph node biopsy (SLNB) was developed as a new modality in the surgical diagnosis of lymph node metastases. Dye and radioisotope are major tracers for the detection of sentinel lymph nodes (SLN). Dye tends to excessively infiltrate into the interstitium due to their small size (less than several nanometers), resulting in difficulties in maintaining clear surgical fields. Radioisotopes are available in limited number of hospitals. Fluorescent nanoparticles are good candidates for SLN tracer to solve these problems, as we can choose suitable particle size and fluorescence wavelength of near-infrared. However, the use of nanoparticles faces safety issues, and many attempts have been performed by givi