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temperature shifts for extraction and purification of zygomycetes chitosan with dilute sulfuric acid温度变化的提取和纯化接合菌壳聚糖与稀硫酸
Int. J. Mol. Sci. 2010, 11, 2976-2987; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN1422-0067 /journal/ijms Article Temperature Shifts for Extraction and Purification of Zygomycetes Chitosan with Dilute Sulfuric Acid Akram Zamani 1,2,*,Lars Edebo 3, Claes Niklasson 2 and Mohammad J. Taherzadeh 1 1 School of Engineering, University of Borås, 50190 Borås, Sweden; E-Mail: Mohammad.Taherzadeh@hb.se 2 Department of Chemical and Biological Engineering, Chalmers University of Technology, 41296 Gothenburg, Sweden; E-Mail: claesn@chalmers.se 3 Department of Clinical Microbiology, University of Gothenburg, 41346 Gothenburg, Sweden; E-Mail: lars.edebo@microbio.gu.se * Author to whom correspondence should be addressed; E-Mail: Akram.Zamani@hb.se; Tel.: +46-33-435-4360; Fax: +46-33-435-4008. Received: 17 July 2010; in revised form: 26 July 2010 / Accepted: 27 July 2010 / Published: 13 August 2010 Abstract: The temperature-dependent hydrolysis and solubility of chitosan in sulfuric acid solutions offer the possibility for chitosan extraction from zygomycetes mycelia and separation from other cellular ingredients with high purity and high recovery. In this study, Rhizomucor pusillus biomass was initially extracted with 0.5 M NaOH at 120 °C for 20 min, leaving an alkali insoluble material (AIM) rich in chitosan. Then, the AIM was subjected to two steps treatme
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