survival and mitochondrial function in septic patients according to mitochondrial dna haplogroup生存和线粒体功能根据线粒体dna haplogroup败血症的病人.pdfVIP

Lorente et al. Critical Care 2012, 16:R10 /content/16/1/R10 RESEARCH Open Access Survival and mitochondrial function in septic patients according to mitochondrial DNA haplogroup 1*† 2† 3 2 4 5 Leonardo Lorente , Ruth Iceta , María M Martín , Esther López-Gallardo , Jordi Solé-Violán , José Blanquer , 6 7 8 2 2,9 Lorenzo Labarta , César Díaz , Alejandro Jiménez , Julio Montoya and Eduardo Ruiz-Pesini Abstract Introduction: We recently found that platelet cytochrome c oxidase (COX) activities and quantities in 6-month- survival septic patients are significantly higher than those of patients who died before 6 months. Other studies suggested that the mitochondrial DNA (mtDNA) genotype could play a major role in sepsis survival. Given that COX catalytic subunits are encoded by mtDNA, the objective of the present study was to explore whether mtDNA population genetic variation could affect COX activity and quantity and favors sepsis survival. Methods: A prospective, multicenter, observational study was carried out in six Spanish ICUs. We included 96 patients with severe sepsis. We determined the mtDNA haplogroup, the COX specific activity/citrate synthase specific activity (COXa/CSa) ratio and the COX quantity/citrate synthase specific activity (COXq/CSa) ratio in circulating platelets at the time of diagnosis, day 4 and day 8. We used survival at 1 and 6 months as endpoints. Results: Patients with the JT mtDNA haplogroup (n = 15) showed higher COXq/CSa ratio at day 4 (P = 0.04) and day 8 (P = 0.02) than those with other haplogroups (n = 81). Logistic regression analysis s