suppressing glucose transporter gene expression in schistosomes impairs parasite feeding and decreases survival in the mammalian host抑制葡萄糖转运体基因表达在血吸虫病损害寄生虫喂养和减少在哺乳动物宿主生存.pdfVIP

Suppressing Glucose Transporter Gene Expression in Schistosomes Impairs Parasite Feeding and Decreases Survival in the Mammalian Host 1 2 1 1 2,3 Greice Krautz-Peterson , Mariana Simoes , Zahra Faghiri , David Ndegwa , Guilherme Oliveira , 1 1 Charles B. Shoemaker , Patrick J. Skelly * 1 Molecular Helminthology Laboratory, Division of Infectious Diseases, Department of Biomedical Sciences, Tufts University, Cummings School of Veterinary Medicine, ´ Grafton, Massachusetts, United States of America, 2 Laboratory of Cellular and Molecular Parasitology and CEBio, Instituto Rene Rachou - FIOCRUZ, Belo Horizonte, MG, ˆ Brazil, 3 Instituto Nacional de Ciencia e Tecnologia em Doenc¸as Tropicais, Salvador, BA, Brazil Abstract Adult schistosomes live in the host’s bloodstream where they import nutrients such as glucose across their body surface (the tegument). The parasite tegument is an unusual structure since it is enclosed not by the typical one but by two closely apposed lipid bilayers. Within the tegument two glucose importing proteins have been identified; these are schistosome glucose transporter (SGTP) 1 and 4. SGTP4 is present in the host interactive, apical tegumental membranes, while SGTP1 is found in the tegumental basal membrane (as well as in internal tissues). The SGTPs act by facilitated diffusion. To examine the importance of these proteins for the parasites, RNAi was employed to knock down expression of both SGTP genes in the schistosomula and adult worm life stage