superhydrophobic poly(l-lactic acid) surface as potential bacterial colonization substrate表面超疏水聚(l-lactic酸)为潜在的细菌殖民化衬底.pdfVIP

Sousa et al. AMB Express 2011, 1:34 /content/1/1/34 ORIGINAL Open Access Superhydrophobic poly(L-lactic acid) surface as potential bacterial colonization substrate 1† 1† 1 1,2,3 1,2,3 1* Cláudia Sousa , Diana Rodrigues , Rosário Oliveira , Wenlong Song , João F Mano and Joana Azeredo Abstract Hydrophobicity is a very important surface property and there is a growing interest in the production and characterization of superhydrophobic surfaces. Accordingly, it was recently shown how to obtain a superhydrophobic surface using a simple and cost-effective method on a polymer named poly(L-lactic acid) (PLLA). To evaluate the ability of such material as a substrate for bacterial colonization, this work assessed the capability of different bacteria to colonize a biomimetic rough superhydrophobic (SH) PLLA surface and also a smooth hydrophobic (H) one. The interaction between these surfaces and bacteria with different morphologies and cell walls was studied using one strain of Staphylococcus aureus and one of Pseudomonas aeruginosa. Results showed that both bacterial strains colonized the surfaces tested, although significantly higher numbers of S. aureus cells were found on SH surfaces comparing to H ones. Moreover, scanning electron microscopy images showed an extracellular matrix produced by P. aeruginosa on SH PLLA surfaces, indicating that this bacterium is able to form a biofilm on such substratum. Bacterial removal through lotus leaf effect was also tested, being more efficient on H coupons than on SH PLLA ones. Overall, the results showed that SH PLLA surfaces can be used as a substrate for bacterial colonization and, thus, have an exceptional potential for bi