studies on the interaction mechanism of pyrene derivatives with human tumor-related dna芘衍生物的相互作用机理研究与人类肿瘤的dna.pdfVIP

Molecules 2012, 17, 14159-14173; doi:10.3390/molecules171214159 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Studies on the Interaction Mechanism of Pyrene Derivatives with Human Tumor-Related DNA Li Li, Jia Lu, Chongzheng Xu, Huihui Li * and Xiaodi Yang * Jiangsu Key Laboratory of New Power Batteries, Jiangsu Key Laboratory of Biofunctional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, China * Authors to whom correspondence should be addressed; E-Mails: huihuili@ (H.L.); yangxiaodi@ (X.Y.); Tel.: +86-25-8359-8648 (H.L.); Fax: +86-25-8589-1767 (H.L.). Received: 22 October 2012; in revised form: 21 November 2012 / Accepted: 26 November 2012 / Published: 28 November 2012 Abstract: Pyrene derivatives can be carcinogenic, teratogenic and mutagenic, thus having the potential to cause malignant diseases. In this work, the interactions of two selected pyrene derivatives (1-OHP and 1-PBO) and human tumor-related DNA (p53 DNA and C-myc DNA) are investigated by spectroscopic and non-native polyacrylamide gel electrophoresis (PAGE) methods. Using fluorescence spectrometry and circular dichroism (CD), DNA interactions of pyrene derivatives are confirmed to occur mainly via the groove binding mode supported by the intercalation into the base pairs of DNA. There is an obvious binding order of pyrene derivatives to the targeted DNA, 1-OHP 1-PBO. The