有哪些信誉好的足球投注网站studies on the interaction mechanism of 1,10-phenanthroline cobalt(ii) complex with dna and preparation of electrochemical dna biosensor研究1的相互作用机理,10-phenanthroline钴(ii)与dna和复杂的电化学dna生物传感器的制备.pdfVIP
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studies on the interaction mechanism of 1,10-phenanthroline cobalt(ii) complex with dna and preparation of electrochemical dna biosensor研究1的相互作用机理,10-phenanthroline钴(ii)与dna和复杂的电化学dna生物传感器的制备
Sensors 2006, 6, 1234-1244 sensors ISSN 1424-8220 © 2006 by MDPI /sensors Full Paper Studies on the Interaction Mechanism of 1,10-Phenanthroline Cobalt(II) Complex with DNA and Preparation of Electrochemical DNA Biosensor Shuyan Niu, Feng Li, Shusheng Zhang*, Long Wang, Xuemei Li and Shiying Wang College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China * Author to whom correspondence should be addressed. E-mail: shushzhang@ Received: 1 September 2006 / Accepted: 5 October 2006 / Published: 10 October 2006 Abstract: Fluorescence spectroscopy and ultraviolet (UV) spectroscopy techniques coupled with cyclic voltammetry (CV) were used to study the interaction between salmon sperm DNA and 1,10-Phenanthroline cobalt(II) complex, [Co(phen) (Cl)(H O)]Cl·H O, where 2 2 2 phen = 1,10-phenanthroline. The interaction between [Co(phen) (Cl)(H O)]+ and double- 2 2 strand DNA (dsDNA) was identified to be intercalative mode. An electrochemical DNA biosensor was developed by covalent immobilization of probe single-strand DNA (ssDNA) related to human immunodeficiency virus (HIV) on the activated glassy carbon electrode (GCE). With [Co(phen) +
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