sonochemically fabricated microelectrode arrays for use as sensing platforms声化学的捏造微电极阵列的传感平台.pdfVIP

Sensors 2010, 10, 5090-5132; doi:10.3390/s100505090 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Review Sonochemically Fabricated Microelectrode Arrays for Use as Sensing Platforms Stuart D. Collyer 1, Frank Davis 2 and Séamus P.J. Higson 2,* 1 Microarray Ltd, PO BOX 88, Manchester, M60 1QD, UK; E-Mail: s.d.collyer@cranfield.ac.uk 2 Cranfield Health, Cranfield University, Cranfield, Bedfordshire, MK43 0AL, UK; E-Mail: f.davis@cranfield.ac.uk * Author to whom correspondence should be addressed; E-Mail: s.p.j.higson@cranfield.ac.uk; Tel.: +44 (0)1234-758516; Fax: +44 (0)1234-758380. Received: 26 January 2010; in revised form: 15 April 2010 / Accepted: 27 April 2010 / Published: 25 May 2010 Abstract: The development, manufacture, modification and subsequent utilisation of sonochemically-formed microelectrode arrays is described for a range of applications. Initial fabrication of the sensing platform utilises ultrasonic ablation of electrochemically insulating polymers deposited upon conductive carbon substrates, forming an array of up to 70,000 microelectrode pores cm–2 . Electrochemical and optical analyses using these arrays, their enhanced signal response and stir-independence area are all discussed. The growth of conducting polymeric ―mushroom‖ protrusion arrays with entrapped biological entities, thereby forming biosensors is detailed. The simplicity and inexpensiveness of this approach, lending itself ideally to mass fabrication cou