purification of chicken carbonic anhydrase isozyme-iii (ca-iii) and its measurement in white leghorn chickens净化的鸡肉碳酸酐酶isozyme-iii(ca-iii)及其测量在白来航鸡鸡.pdfVIP

Nishita et al. Acta Veterinaria Scandinavica 2011, 53:63 /content/53/1/63 RESEARCH Open Access Purification of chicken carbonic anhydrase isozyme-III (CA-III) and its measurement in White Leghorn chickens 1* 2 1 3 4 5 Toshiho Nishita , Yuichiro Tomita , Daisuke Yorifuji , Kensuke Orito , Hideharu Ochiai and Kazuyosi Arishima Abstract Background: The developmental profile of chicken carbonic anhydrase-III (CA-III) blood levels has not been previously determined or reported. We isolated CA-III from chicken muscle and investigated age-related changes in the levels of CA-III in blood. Methods: CA-III was purified from chicken muscle. The levels of CA-III in plasma and erythrocytes from 278 female chickens (aged 1-93 weeks) and 68 male chickens (aged 3-59 weeks) were determined by ELISA. Results: The mean level of CA-III in female chicken erythrocytes (1 week old) was 4.6 μg/g of Hb, and the CA-III level did not change until 16 weeks of age. The level then increased until 63 weeks of age (11.8 μg/g of Hb), decreased to 4.7 μg/g of Hb at 73 weeks of age, and increased again until 93 weeks of age (8.6 μg/g of Hb). The mean level of CA-III in erythrocytes from male chickens (3 weeks old) was 2.4 μg/g of Hb, and this level remained steady until 59 weeks of age. The mean plasma level of CA-III in 1-week-old female chickens was 60 ng/mL, and this level was increased at 3 weeks of age (141 ng/mL) and then remained steady until 80 weeks of age (122 ng/ mL). The mean plasma level of CA-III in 3-week-old male chickens was 58 ng/mL, and this level remained steady until 59 weeks of age. Conclusion: We observed both developmental changes and sex differences in CA-III concentratio