人参皂苷刺激西洋参土传病原菌的生长.pdfVIP

Phytochemistry 64 (2003) 257–264 /locate/phytochem Ginsenosides stimulate the growth of soilborne pathogens of American ginseng a a b a, Robert W. Nicol , Lina Yousef , James A. Traquair , Mark A. Bernards * aDepartment of Biology, University of Western Ontario, London, ON, Canada N6A 5B7 bSCPFRC, Agriculture and Agri-Food Canada, London, ON, Canada N5V 4T3 Received 16 January 2003; received in revised form 22 April 2003 Dedicated to the memory of Professor Jeffrey B. Harborne Abstract Ginseng saponins (ginsenosides) were isolated from soil associated with the roots of commercially grown American ginseng (Panax quinquefolius L.), identified via LC-MS and quantified via analytical HPLC. The ginsenosides, including F , Rb , Rb , Rc, 11 1 2 Rd, Re and Rg1, represented between 0.02 and 0.098% (average 0.06%) of the mass of the soil collected from roots annually between 1999 and 2002. The same ginsenosides were also isolated from run-off of undisturbed plants grown in pots in a greenhouse using a root exudate trapping system. To investigate (1) whether these saponins could influence the growth of pythiaceous fungi pathogenic to ginseng, and (2) whether soil levels of ginsenosides were sufficient to acc