高脂食物促进胶原诱导关节炎大鼠的关节损害-中国试验动物学报.docVIP

高脂饲料对胶原诱导关节炎大鼠关节的影响 陈哲1，杨思思2，燕政寰3，刘亚飞1，王玉1，涂胜豪1 (1.华中科技大学同济医学院附属同济医院中西医结合科，2.华中科技大学同济医学院附属同济医院综合科，3.华中科技大学同济医学院1106班，武汉 430030) 【摘要】目的 通过比较不同饮食对胶原诱导关节炎(collagen-induced arthritis，CIA)大鼠关节的影响，明确高脂饲料对关节炎的作用。 方法 选用Wistar大鼠建立关节炎模型，给予普通和高脂饲料，观察不同饮食条件下大鼠关节炎指数积分、滑膜HE染色以及检测血清中血脂和炎症因子的浓度。结果 与普通饲料CIA大鼠组比较，高脂饲料的CIA大鼠关节炎指数积分增加且持续加重，病理切片的滑膜增生层次、血管翳形成和炎性细胞浸润均明显增高，高脂饲料CIA组血清中TC、LDL水平明显升高（P0.0），而HDL则明显降低（P0.05），前炎症因子TNF-α、IL-1β和IL-17的浓度也明显升高（P0.05）。结论 高脂饲料可能通过增强滑膜增生和促进炎症因子来促进CIA关节炎的进展。 【关键词】高脂饲料；胶原诱导关节炎；Wistar大鼠 【中图分类号】R332 【文献标识码】 【文章编号】 The Effects of High Fat Diet on Joints of Collagen-induced Arthritis Rats CHEN Zhe1，YANG Si-si2，YAN Zheng-Huan3，LIU Ya-fei1，WANG Yu1，TU Sheng-hao1 Department of Integrated Chinese Traditional and Western Medicine, Tongji Hospital 2.Department of Geriatrics, Tongji Hospital 3. Class 1106 of Tongji Medical College, Huazhong University of Science and Technology，Wuhan 430030，China) 【Abstract】 Objective To study the effects of high fat diet on joints of collagen-induced arthritis rats by comparing different diets. Methods After the model of Wistar CIA rats was established, We feed normal and high fat diet. The arthritis index scores were measured and the synovium were examined with HE stain and the concentrations of lipids and inflammatory factors in serum were detected. Results Compared with those in normal group, the arthritis index scores, synovial proliferation, pannus formation and inflammatory cell infiltration in synovium HE stain were increased in CIA rats with high fat diet. Plasma levels of TC and LDL were also enhanced in high fat diet CIA rats group(P0.01) apart from HDL, which were decreased obviously(P0.05). Plasma levels of Pro-inflammatory cytokines,that is TNF-α, IL-1β and IL-17 were also significantly increased in high fat diet CIA rats(P0.05) . Conclusions High fat diet may promote the progress of arthritis in CIA rats by enhancing synovial hyperplasia and inflammatory cytokines. 【Key words】H