几种抗肿瘤药物诱导Jurkat细胞凋亡探究.docVIP

几种抗肿瘤药物诱导Jurkat细胞凋亡探究 　【摘要】 　　本研究评价几种临床常用的抗肿瘤药物顺铂（CDDP）、羟基喜树碱(HCPT)、高三尖杉酯碱(HHT)和米托蒽醌(MIT)在白血病治疗中的作用，并分析其在诱导Jurkat细胞凋亡的作用，为白血病的临床治疗提供新的理论依据和思路。用Annexin V/PI双参数流式分析方法，检测这几种抗肿瘤药物诱导Jurkat细胞凋亡和死亡的效应。结果表明：MIT和HCPT在加药处理早期4小时 (Plt;0.05)和后期第8小时(Plt;0.05)都有明显的诱导Jurkat细胞凋亡的效应。HHT有明显诱导Jurkat细胞凋亡的效果，但各剂量组之间无显著差异。CDDP在高浓度和长时间作用时才显示较弱的细胞凋亡效应，明显弱于前3者。各种药物作用后均未观察到明显的细胞死亡效应。结论：MIT诱导Jurkat细胞凋亡的作用最显著，Annexin V流式分析方法可作为一种可靠的临床药物筛选的方法。 【关键词】 抗癌药物；细胞凋亡；Jurkat细胞系；Annexin V 　　Effect of Several Anti-tumor Drugs on Apoptosis Induction in Jurkat Cell Line 　　Abstract Cisplatin (CDDP)，homoharringtonine(HHT)，mitoxantrone(MIT) and hydroxycamptothecin(HCPT) are highly effective anti-tumor drugs. To evaluate their effects in the therapy of leukemia and establish a valuable method to estimate anti-tumor drugs，Annexin V/PI double parameter flow cytometry was used to detect the effects of these drug inducing apoptosis and death in Jurkat cell line. The results showed that MIT and HCTP-induced apoptosis effects on Jurkat cell line were obvious at 4 hours in early phase after adding drug (Plt;0.05) and at 8 hours in late phase after adding drug (Plt;0.05). HHT had obvious effect on inducing apoptosis of Jurkat cells，but no significant difference from low to high doses. The effect of CDDP on inducing apoptosis of Jurkat cell line was obviously weaker than that of HHT，MIT and HCPT，its weak effect on apoptosis of Jurkat cell line was found only at high concentration of drug for long time. Death effects on Jurkat cell line can not be observed in every experimental group. It is concluded that low dose of MIT can effectively induced apoptosis of Jurkat cell line. Annexin V/PI double parameter flow cytometry can be used as a reliable method for clinical screening anti-tumor drugs. 　　Key words anti-tumor drug; apoptosis; Jurkat cell line; Annexin V 化疗是肿瘤治疗的一种重要方法，新的抗肿瘤药物也不断地涌现。国内在上个世纪90年代上市的抗癌药物中化学合成药米托蒽醌(MIT)、羟基脲和顺铂(CDDP)等少数几种抗癌药物，通过与DNA分子结合抑制核酸合成引起细胞死亡，这类药物称之为细胞周期非特异性药物。本研究选用米托蒽醌(MIT)、顺铂(CDDP)、羟基喜树碱(HCPT)、 高