基于印迹聚吡咯膜电化学传感器检测S-卵白蛋白的研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2016, Vol.32, No.12 基于印迹聚吡咯膜电化学传感器检测S-卵白蛋白 的研究 马美湖，曾齐 （国家蛋品加工技术研发分中心，华中农业大学食品科技学院，湖北武汉 430070 ） 摘要：鉴于蛋清中S-卵白蛋白的含量与鸡蛋新鲜度和品质有较大相关性，将S-卵白蛋白含量作为评价鸡蛋新鲜度和品质的指标 具有较大的前景和意义。本文通过循环伏安法和差分脉冲伏安法对传感器制备的扫描圈数、扫描速率、模板洗脱时间和孵化时间进行 了优化，确定最佳扫描圈数为18，扫描速率为100 mV/s，模板洗脱时间为1.5 h，孵化时间为40 min ，成功制备了能特异性识别S- 卵白蛋白的印迹聚吡咯膜电化学传感器。采用场发射扫描电镜对印迹和非印迹传感器的微观结构进行了表征。通过差分脉冲伏安法， -6 -3 探讨了不同S-卵白蛋白浓度与峰电流变化的依赖关系，确定S-卵白蛋白浓度在10 ~ 10 mg/mL 范围内时，浓度与峰电流变化值具有 良好线性关系，并得出检出限为6.75×10-7 mg/mL 。该印迹传感器对S-卵白蛋白具有良好特异性性响应，可用于定量检测S-卵白蛋白。 关键词：蛋清；S-卵白蛋白；聚吡咯；分子印迹；电化学传感器 文章篇号：1673-9078(2016)12-329-336 DOI: 10.13982/j.mfst.1673-9078.2016.12.050 Electrochemical Sensor Based on Molecularly Imprinted Polypyrrole Film for Detection of S-ovalbumin MA Mei-hu, ZENG Qi (National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China) Abstract: Since the S-ovalbumin content in egg white has a significant correlation with the freshness and quality of chicken eggs, S-ovalbumin can be consider as a useful indicator for the same. Conditions were optimized using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) to prepare a molecularly-imprinted polypyrrole (MIPs) sensor that selectively recognizes S-ovalbumin. The optimal scan cycles, scan rate, template extraction time, and incubation time were 18 cycles, 100 mV/s, 1.5 h, and 40 min, respectively. The micro-structure of MIPs and non-MIPs were characteri