nod／scid小鼠异种急性移植物抗宿主病模型的-中国试验动物学报.docVIP

人脐带间充质干细胞静脉输注小鼠的安全性 何君1,2，李洋1,2，陈威1,2，郝好杰3，李名烁1,2，韩瑞红1,2，1,2，卢星辰1,2，金翠英1,2，郭珣1,2，易辉1,2，李晓岑1,2，赵颖1,2，武岩1,2，徐玉环1,2 1.中国医学科学院医学实验动物研究所，北京，100021中国医学科学院糖尿病研究中心，北京，100005解放军总医院生命科学院基础医学所北京，100 【摘要】目的 探讨C57/BL6J小鼠重复多次尾静脉输注人脐带间充质干细胞后的免疫反应和毒性。方法 将SPF级别的32只C57/BL6J小鼠随机分为阴性对照组、细胞移植组，每组16 只，雌雄各半，细胞移植组小鼠尾静脉注射分离培养的第5代人脐带间充质干细胞，一次5×106/只，每周注射一次，连续注射4周；阴性对照组每次注射相同容积的PBS。注射后后观察小鼠的一般症状，末次注射后1周、4周进行血细胞计数、血生化、免疫反应指标、脏器质量测定和组织病理学检查。结果 细胞移植组小鼠血细胞计数、血生化、脏器重量和脏器系数与对照组无显著性差异（P 0.05），脏器组织病理学在光镜下检查结果与对照组无形态学差别，以及免疫结果测定（T细胞亚群CD3+、CD4、CD8CD4+/CD8+）与对照组( P 0.05)，较对照组有显著性差异（P(0.01） 结论中图分类号 A safety study of intravenous injection of human umbilical cord mesenchymal stem cells in mice HE Jun1,2, LI Yang1,2, CHEN Wei1,2, HAO Hao-jie3, LI Ming-shuo1,2, HAN Rui-hong1,2, WU Xin1,2, LU Xing-chen1,2, JIN Cui-ying1,2, GUO Xun1,2, YI Hui1,2, LI Xiao-cen1,2, ZHAO Ying1,2, WU Yan1,2, XU Yu-huan1,2, 1.Institute of Laboratory Animal Science，Chinese Academy of Medical Science，Beijing 100021，China Diabetes research center，Chinese Academy of Medical Science，Beijing 100005，ChinaInstitute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital，Beijing 100，China[Abstract] Objective: To study the toxicity of intravenous injection of human umbilical cord mesenchymal stem cells in C57/BL6J mice with repeated administrations. Methods: 32 SPF C57/BL6J mice were randomly divided into negative control group and HUMSC transplantation group, of equal gender. HUMSCs were isolated and subsequently cultured through 5 passages in vitro. 2×106 HUMSCs were injected each mice of transplantation group via tail vein once 1 week for 4 weeks, and the equal quantity PBS were injected to control group mice. Mice were euthanatized on the day of 1 week (8 mice per group) and 4 weeks(all survived mice) after the last treatment following the schedule. Toxicity was evaluated by clinical observations, pathology (blood cell counts, clinical biochemistry), immunologic consequences, visceral organs weight and anato