antibiofilm activity of an exopolysaccharide from marine bacterium vibrio sp. qy101antibiofilm活动从海洋细菌胞外多糖的弧菌qy101 sp.pdfVIP

Antibiofilm Activity of an Exopolysaccharide from Marine Bacterium Vibrio sp. QY101 Peng Jiang1,2,3., Jingbao Li1,2,3,4., Feng Han1,2,3, Gaofei Duan1,2,3, Xinzhi Lu1,2,3, Yuchao Gu1,2,3, Wengong Yu1,2,3* 1 Key Laboratory of Marine Drugs, Chinese Ministry of Education, Ocean University of China, Qingdao, People’s Republic of China, 2 Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Ocean University of China, Qingdao, People’s Republic of China, 3 Laboratory of Glycobiology, School of Medicine and Pharmacy, Ocean University of China, Qingdao, People’s Republic of China, 4 Key Laboratory For Space Bioscience and Biotechnology, Faculty of Life Sciences, Northwestern Polytechnical University, Xi’an, People’s Republic of China Abstract Bacterial exopolysaccharides have always been suggested to play crucial roles in the bacterial initial adhesion and the development of complex architecture in the later stages of bacterial biofilm formation. However, Escherichia coli group II capsular polysaccharide was characterized to exert broad-spectrum biofilm inhibition activity. In this study, we firstly reported that a bacterial exopolysaccharide (A101) not only inhibits biofilm formation of many bacteria but also disrupts established biofilm of some strains. A101 with an average molecular weight of up to 546 KDa, was isolated and purified from the culture supernatant of the marine bacterium Vibrio sp. QY101 by ethanol precipitation, iron-exchange chromatography and gel filtration chromatography. High performance liquid chromatography traces of the hydrolyzed polysaccharides showed that A101 is primarily consisted of galacturonic acid, glucuronic acid, rhamnose and glucosamine. A101 was demonstrated to inhibit biofilm formation by a wide range of Gram-negative and Gram-positive bacteria with