an assessment of the role of dna adenine methyltransferase on gene expression regulation in e coli评估的作用dna腺嘌呤甲基转移酶在大肠杆菌基因表达调控.pdfVIP

An Assessment of the Role of DNA Adenine Methyltransferase on Gene Expression Regulation in E coli Aswin Sai Narain Seshasayee* Genomics and Regulatory Systems Group, EMBL-European Bioinformatics Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom N6-Adenine methylation is an important epigenetic signal, which regulates various processes, such as DNA replication and repair and transcription. In c-proteobacteria, Dam is a stand-alone enzyme that methylates GATC sites, which are non- randomly distributed in the genome. Some of these overlap with transcription factor binding sites. This work describes a global computational analysis of a published Dam knockout microarray alongside other publicly available data to throw insights into the extent to which Dam regulates transcription by interfering with protein binding. The results indicate that DNA methylation by DAM may not globally affect gene transcription by physically blocking access of transcription factors to binding sites. Down-regulation of Dam during stationary phase correlates with the activity of TFs whose binding sites are enriched for GATC sites. Citation: Seshasayee ASN (2007) An Assessment of the Role of DNA Adenine Methyltransferase on Gene Expression Regulation in E ?coli. PLoS ONE 2(3): e273. doi:10.1371/journal.pone.0000273 INTRODUCTION identify differentially expressed genes [10]. For all microarray data Dam is an N6-Adenine methyltransferase, which methylates except the dam mutant, differential expression was defined by a q- GATC sites soon after replication. Methylation is a bacterial value of 0.05 following FDR multiple testing. For the dam mutant, version of an immune response to phages. It has been described as the cutoff was 0.01 without multiple