a large insertion in bhlh transcription factor brtt8 resulting in yellow seed coat in brassica rapa一个大型插入bhlh转录因子brtt8导致黄色芸苔属植物的种子外衣拉伯.pdfVIP

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a large insertion in bhlh transcription factor brtt8 resulting in yellow seed coat in brassica rapa一个大型插入bhlh转录因子brtt8导致黄色芸苔属植物的种子外衣拉伯.pdf

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a large insertion in bhlh transcription factor brtt8 resulting in yellow seed coat in brassica rapa一个大型插入bhlh转录因子brtt8导致黄色芸苔属植物的种子外衣拉伯

A Large Insertion in bHLH Transcription Factor BrTT8 Resulting in Yellow Seed Coat in Brassica rapa 1 2 1 3 4 1 1 1 1 Xia Li , Li Chen , Meiyan Hong , Yan Zhang , Feng Zu , Jing Wen , Bin Yi , Chaozhi Ma , Jinxiong Shen , Jinxing Tu1*, Tingdong Fu1 1 National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, Wuhan, P.R. China, 2 Guizhou Rapeseed Institute, Guizhou Academy of Agricultural Sciences, Guiyang, P.R. China, 3 Vegetable Research Institute, Guangdong Academy of Agriculture Sciences, Guangdong, P.R. China, 4 Industrial Crop Research Institute, Yunnan Academy of Agricultural Science, Kunming, P.R. China Abstract Yellow seed is a desirable quality trait of the Brassica oilseed species. Previously, several seed coat color genes have been mapped in the Brassica species, but the molecular mechanism is still unknown. In the present investigation, map-based cloning method was used to identify a seed coat color gene, located on A9 in B. rapa. Blast analysis with the Arabidopsis genome showed that there were 22 Arabidopsis genes in this region including at4g09820 to at4g10620. Functional complementation test exhibited a phenotype reversion in the Arabidopsis thaliana tt8-1 mutant and yellow-seeded plant. These results suggested that the candidate gene was a homolog of TRANSPARENT TESTA8 (TT8) locus. BrTT8 regulated the accumulation of proanthocyanidins (PAs) in the seed coat. Sequence analysis of two alleles revealed a large insertion of a new class of transposable elements, Helitron in yellow sarson . In addition, no mRNA expression of BrTT8 was detected in the yellow-seeded line. It indicated that the natural