a comprehensive analysis of gene expression changes provoked by bacterial and fungal infection in c. elegans综合分析基因表达变化引起的细菌和真菌在秀丽隐杆线虫感染.pdfVIP

A Comprehensive Analysis of Gene Expression Changes Provoked by Bacterial and Fungal Infection in C. elegans 1,2,3 ´ 1,2,3 4 ´ ´ ˜ 1,2,3 Ilka Engelmann , Aurelien Griffon , Laurent Tichit , Frederic Montanana-Sanchis , Guilin 5 5 6 6 1,2,3 Wang , Valerie Reinke , Robert H. Waterston , LaDeana W. Hillier , Jonathan J. Ewbank * ´ ´ ´ 1 Centre d’Immunologie de Marseille-Luminy, Universite de la Mediterranee, Marseille, France, 2 INSERM, U631, Marseille, France, 3 CNRS, UMR6102, Marseille, France, ´ 4 Institut de Mathematiques de Luminy, Marseille, France, 5 Department of Genetics, Yale University School of Medicine, New Haven, Connecticut, United States of America, 6 Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, United States of America Abstract While Caenorhabditis elegans specifically responds to infection by the up-regulation of certain genes, distinct pathogens trigger the expression of a common set of genes. We applied new methods to conduct a comprehensive and comparative study of the transcriptional response of C. elegans to bacterial and fungal infection. Using tiling arrays and/or RNA- sequencing, we have characterized the genome-wide transcriptional changes that underlie the host’s response to infection by three bacterial (Serratia marcescens, Enterococcus faecalis and otorhabdus luminescens) and two fungal path