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a centrifugation-based method for preparation of gold nanoparticles and its application in biodetectioncentrifugation-based方法制备金纳米粒子在biodetection及其应用
Int. J. Mol. Sci. 2007, 8, 526-532
International Journal of
Molecular Sciences
ISSN 1422-0067
© 2007 by MDPI
/ijms
Full Research Paper
A Centrifugation-based Method for Preparation of Gold
Nanoparticles and its Application in Biodetection
Zhiqiang Liang 1,2, Juan Zhang 2, Lihua Wang *,2, Shiping Song 2, Chunhai Fan 2 and
Genxi Li *,1
1 Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing
University, Nanjing 210093, China; E-mail: suyuanyuan@ (Zhiqiang Liang);
genxili@ (Genxi Li)
2 Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;
E-mail: suyuanyuan@ (Juan Zhang); wanglihua@ (Lihua Wang);
songshiping@ (Shiping Song); fchh@ (Chunhai Fan);
* Authors to whom correspondence should be addressed; E-mails: genxili@,
wanglihua@
Received: 18 April 2007; in Revised Form: 11 May 2007 / Accepted: 15 May 2007 /
Published: 13 June 2007
Abstract: Gold nanoparticles (AuNPs) have found widespread applications in life
sciences. While synthesis of monodispersed AuNPs has been fairly convenient by using
chemical reduction of chloroauric acid by sodium citrate, we found that AuNPs of high
quality and high concentrations were not readily obtained via this method. As an example,
we showed that monodispersed 13-nm AuNPs were readily synthesized at relatively low
concentrations (e.g. 3.5 nM); in contrast, 13
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