14-3-3ε is required for germ cell migration in drosophila14-3-3ε需要在果蝇生殖细胞迁移.pdfVIP

14-3-3e Is Required for Germ Cell Migration in Drosophila ¤ K. Kirki Tsigkari, Summer F. Acevedo , Efthimios M. C. Skoulakis* Biomedical Sciences Research Centre ‘‘Alexander Fleming’’, Vari, Greece Abstract Although 14-3-3 proteins participate in multiple biological processes, isoform-specific specialized functions, as well as functional redundancy are emerging with tissue and developmental stage-specificity. Accordingly, the two 14-3-3e proteins in Drosophila exhibit functional specificity and redundancy. Homozygotes for loss of function alleles of D14-3-3e contain significantly fewer germ line cells (pole cells) in their gonads, a phenotype not shared by mutants in the other 14-3-3 gene leo. We show that although D14-3-3e is enriched within pole cells it is required in mesodermal somatic gonad precursor cells which guide pole cells in their migration through the mesoderm and coalesce with them to form the embryonic gonad. Loss of D14-3-3e results in defective pole cell migration, reduced pole cell number. We present evidence that D14-3-3e loss results in reduction or loss of the transcription factor Zfh-1, one of the main regulatory molecules of the pole cell migration, from the somatic gonad precursor cells. Citation: Tsigkari KK, Acevedo SF, Skoulakis EMC (2012) 14-3-3e Is Required for Germ Cell Migration in Drosophila. PLoS ONE 7(5): e36702. doi:10.1371/ journal.pone.0036702 Editor: Christos Samakovlis, Stockholm University, Sweden Received January 11, 2012; Accepted April 5, 2012; Published May 30, 2012 Copyright: 2012 Tsigkari et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are