具有去除染发剂色素功能之土壤细菌筛选与鉴定-建国科技大学.pdfVIP

具有去除染髮劑色素功能之土壤細菌篩選與鑑定 Isolation and Identification of hair dye decolorization Soil Bacteria 蕭蓉禎 Rong-Jen Shiau 摘要 隨著國人生活水準提昇，美容、美髮業發展迅速，美容美髮各項產品使用量大幅提高。然而 大量使用美妝產品（例如：染髮劑等），其污水若未經適當處理，則會對環境造成極大的危害。 本研究旨在篩選具有去除染髮劑色素功能之土壤細菌。利用市售染髮劑做為基質，以增殖培養法 (enrichment culture )篩選土壤細菌，獲得B16以及B21二株具有移除溶液中染髮劑色素能力的細菌 。 將菌株的 16S rRNA基因定序後，與基因庫中的已知菌株的 16S rRNA基因序列進行比對，結果顯 示B16以及B21與Pseudomonas putida非常相近。本研究所分離的菌株未來可應用於美髮業污水處理 減少美髮業污水對環境的危害。 關鍵詞：染髮劑色素 、去除色素、土壤細菌、增殖培養 Abstract As raising the level of life, beauty and hairdressing industries are developing rapidly. However, the waste water of beauty products (such as hair dyes) might harm to the environment. The purpose of this study was to discover soil bacteria which can remove hair dye pigments from aquatic solutions. Using a commercial hair dye as matrix, the enrichment culture method was applied to obtain bacteria. Two bacterial strains were isolated, named B16 and B21. The sequences of 16S rRNA of these two bacteria were submitted to Genbank for species identification. The result showed that both B16 and B21 were highly similar to 1 Pseudomonas putida . These two bacteria could be used for removing hair dye pigments for the hairdressing industry. Keywords: hair dye; decolorization; soil bacteria; enrichment culture 2 蕭蓉禎 博士 建國科技大學美容系 Department of Beauty Science, Chienkuo Technology University 彰化市介壽北路1號 Tel: 04-7111111#2628 E-mail: rjshiau@.tw 3 壹、前言 人類在很早之前就開始使用染料塗抹身體和頭髮。在 4000 年前的古埃及，埃及人就已經利 用天然植物和礦物將木乃伊的手指和腳趾著色，直到今日 ，染料的利用已遍及我們每日所需的 日常生活用品。 現今的市售染髮劑種類繁多 ，依據其化學成份 ，可分為以下四種 (Zviak and Millequant, 2005) ： （一）氧化（永久性）染髮劑[Oxidati