DETERMINATION OF THE SEMISYNTHETIC （半合成的决心）.pdfVIP

Not to be quoted without prior reference to the authors Fisheries Research Services Contract Report No 01/04 DETERMINATION OF THE SEMI-SYNTHETIC AVERMECTIN, EMAMECTIN BENZOATE, BY LIQUID CHROMATOGRAPHY – ATMOSPHERIC PRESSURE CHEMICAL IONISATION MASS SPECTROMETRY (LC-APCI-MS) IN SEDIMENT FROM LOCH SUNART AND LOCH KISHORN Pamela Walsham and Lynda Webster January 2004 Fisheries Research Services Marine Laboratory Victoria Road Aberdeen AB11 9DB DETERMINATION OF THE SEMI-SYNTHETIC AVERMECTIN, EMAMECTIN BENZOATE, BY LIQUID CHROMATOGRAPHY – ATMOSPHERIC PRESSURE CHEMICAL IONISATION MASS SPECTROMETRY (LC-APCI-MS) IN SEDIMENT FROM LOCH SUNART AND LOCH KISHORN Pamela Walsham and Lynda Webster Fisheries Research Services, Marine Laboratory 375 Victoria Road, Aberdeen, AB11 9DB SUMMARY 1. In 2000 the UK Veterinary Medicines Directorate (VMD) issued a Marketing Authorisation for the treatment of sea lice in salmon aquaculture using Slice® , which has the active ingredient emamectin benzoate. 2. A liquid chromatography – atmospheric pressure chemical ionisation mass spectrometry (LC-APCI-MS) method was developed and validated for the determination of the semi- synthetic avermectin, emamectin benzoate, in marine sediment. Recoveries from sediment samples ranged from 66.50% to 102.50% (mean = 85.7%, n= 7, standard deviation [SD] = 11.97). 3. A total of thirty-two sediment samples were collected from Loch Kishorn and Loch Sunart and analysed for emamectin benzoate. Sediment samples were collect