genomic sequence around butterfly wing development genes annotation and comparative analysis基因组序列在蝴蝶翅膀开发基因注释和比较分析.pdfVIP

Genomic Sequence around Butterfly Wing Development Genes: Annotation and Comparative Analysis ˆ ˜ 1 2 2¤ ´ 1,3 Ines C. Conceic¸ao , Anthony D. Long , Jonathan D. Gruber , Patrıcia Beldade * ˆ 1 Instituto Gulbenkian de Ciencia, Oeiras, Portugal, 2 University of California Irvine, Irvine, California, United States of America, 3 Institute of Biology, Leiden University, Leiden, The Netherlands Abstract Background: Analysis of genomic sequence allows characterization of genome content and organization, and access beyond gene-coding regions for identification of functional elements. BAC libraries, where relatively large genomic regions are made readily available, are especially useful for species without a fully sequenced genome and can increase genomic coverage of phylogenetic and biological diversity. For example, no butterfly genome is yet available despite the unique genetic and biological properties of this group, such as diversified wing color patterns. The evolution and development of these patterns is being studied in a few target species, including Bicyclus anynana, where a whole-genome BAC library allows targeted access to large genomic regions. Methodology/Principal Findings: We characterize ,1.3 Mb of genomic sequence around 11 selected genes expressed in B. anynana developing wings. Extensive manual curation of in silico predictions, also making use of a large dataset of expressed genes for this species, identified repetitive elements and protein coding sequence, and highlighted an expansion of Alcohol dehydrogenase genes. Comparative analysis with orthologous regions of the lepidopteran refere