gene expression disruptions of organism versus organ in drosophila species hybrids生物的基因表达中断和器官在果蝇物种混合动力车.pdfVIP

Gene Expression Disruptions of Organism versus Organ in Drosophila Species Hybrids Daniel J. Catron, Mohamed A. F. Noor* Biology Department, Duke University, Durham, North Carolina, United States of America Abstract Hybrid dysfunctions, such as sterility, may result in part from disruptions in the regulation of gene expression. Studies of hybrids within the Drosophila simulans clade have reported genes expressed above or below the expression observed in their parent species, and such misexpression is associated with male sterility in multigenerational backcross hybrids. However, these studies often examined whole bodies rather than testes or had limited replication using less-sensitive but global techniques. Here, we use a new RNA isolation technique to re-examine hybrid gene expression disruptions in both testes and whole bodies from single Drosophila males by real-time quantitative RT-PCR. We find two early-spermatogenesis transcripts are underexpressed in hybrid whole-bodies but not in assays of testes alone, while two late-spermatogenesis transcripts seem to be underexpressed in both whole-bodies and testes alone. Although the number of transcripts surveyed is limited, these results provide some support for a previous hypothesis that the spermatogenesis pathway in these sterile hybrids may be disrupted sometime after the expression of the early meiotic arrest genes. Citation: Catron DJ, Noor MAF (2008) Gene Expression Disruptions of Organism versus Organ in Drosophila Species Hybrids. PLoS ONE 3(8): e3009. doi:10.1371/ journal.pone.0003009 Editor: Adam K. Chippindale, Queens University, Canada Received June 11, 2008; Accepted July 31, 2008; Published August 20, 2008 Copyright: 2008 Catron et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, d