gene atlasing of digestive and reproductive tissues in schistosoma mansoni基因图谱的消化道和生殖组织在曼氏裂体吸虫.pdfVIP

Gene Atlasing of Digestive and Reproductive Tissues in Schistosoma mansoni 1,2 1 1 1,2. Sujeevi S. K. Nawaratna , Donald P. McManus , Luke Moertel , Geoffrey N. Gobert , Malcolm K. 1,2 . Jones * 1 Queensland Institute of Medical Research, Herston, Australia, 2 School of Veterinary Sciences, The University of Queensland, Gatton, Australia Abstract Background: While considerable genomic and transcriptomic data are available for Schistosoma mansoni, many of its genes lack significant annotation. A transcriptomic study of individual tissues and organs of schistosomes could play an important role in functional annotation of the unknown genes, particularly by providing rapid localisation data and thus giving insight into the potential roles of these molecules in parasite development, reproduction and homeostasis, and in the complex host-parasite interaction. Methodology/Principal Findings: Quantification of gene expression in tissues of S. mansoni was achieved by a combination of laser microdissection microscopy (LMM) and oligonucleotide microarray analysis. We compared the gene expression profile of the adult female gastrodermis and male and female reproductive tissues with whole worm controls. The results revealed a total of 393 genes (contigs) that were up-regulated two-fold or more in the gastrodermis, 4,450 in the ovary, 384 in the vitelline tissues of female parasites, and 2,171 in the testes. We have also supplemented these data with the identification of highly expressed genes in different regions of manually dissected male and female S. mansoni. Though relatively crude, this dissection strategy provides low resolution localisation data for criti