fluorescence behavioral imaging (fbi) tracks identity in heterogeneous groups of drosophila荧光行为成像(fbi)跟踪身份异构组果蝇.pdfVIP

Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila 1,2 2 2 1 Pavan Ramdya *, Thomas Schaffter , Dario Floreano , Richard Benton 1 Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland, 2 Laboratory of Intelligent Systems, Institute of ´ ´ ´ Microengineering, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland Abstract Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila. Citation: Ramdya P, Schaffter T, Floreano D, Benton R (2012) Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila. PLoS ONE 7(11): e48381. doi:10.1371/journal.pone.0048381 Editor: Matthieu Louis, Center for Genomic Regulation, Spain Received May 14, 2012; Accepted September 25, 2012; Published November 7, 2012 Copyright: 2012 Ramdya et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, a