fli+ etsrp+ hemato-vascular progenitor cells proliferate at the lateral plate mesoderm during vasculogenesis in zebrafishfli + etsrp + hemato-vascular祖细胞增殖在侧板中胚层在斑马鱼血管生成.pdfVIP

Fli + etsrp+ Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish ¤ Chang Zoon Chun*, Indu Remadevi, Marcus-Oliver Schupp, Ganesh Vinayak Samant, Kallal Pramanik , George Albert Wilkinson, Ramani Ramchandran* Department of Pediatrics, CRI Developmental Vascular Biology Program, Translational and Biomedical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America Abstract Background: Vasculogenesis, the de novo formation of blood vessels from precursor cells is critical for a developing embryo. However, the signals and events that dictate the formation of primary axial vessels remain poorly understood. Methodology/Principal Findings: In this study, we use ets-related protein-1 (etsrp), which is essential for vascular development, to analyze the early stages of vasculogenesis in zebrafish. We found etsrp+ cells of the head, trunk and tail follow distinct developmental sequences. Using a combination of genetic, molecular and chemical approaches, we demonstrate that fli +etsrp+ hemato-vascular progenitors (FEVPs) are proliferating at the lateral plate mesoderm (LPM). The Shh-VEGF-Notch-Hey2 signaling pathway controls the proliferation process, and experimental modulation of single components of this pathway alters etsrp+ cell numbers at the LPM. Conclusions/Significance: This study for the first time defines factors controlling proliferation, and cell numbers of pre- migratory FEVPs in zebrafish. Citation: Chun CZ, Remadevi I, Schupp M-O, Samant GV, Pramanik K,