a rapid and economic in-house dna purification method using glass syringe filters快速和经济内部dna纯化方法使用玻璃注射器过滤器.pdfVIP

A Rapid and Economic In-House DNA Purification Method Using Glass Syringe Filters ¤ Yun-Cheol Kim *, Sherie L. Morrison Department of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, California, United States of America Abstract Background: Purity, yield, speed and cost are important considerations in plasmid purification, but it is difficult to achieve all of these at the same time. Currently, there are many protocols and kits for DNA purification, however none maximize all four considerations. Methodology/Principal Findings: We now describe a fast, efficient and economic in-house protocol for plasmid preparation using glass syringe filters. Plasmid yield and quality as determined by enzyme digestion and transfection efficiency were equivalent to the expensive commercial kits. Importantly, the time required for purification was much less than that required using a commercial kit. Conclusions/Significance: This method provides DNA yield and quality similar to that obtained with commercial kits, but is more rapid and less costly. Citation: Kim Y-C, Morrison SL (2009) A Rapid and Economic In-House DNA Purification Method Using Glass Syringe Filters. PLoS ONE 4(11): e7750. doi:10.1371/ journal.pone.0007750 Editor: Carles Lalueza-Fox, Institute of Evolutionary Biology (CSIC-UPF), Spain Received August 13, 2009; Accepted September 25, 2009; Published November 18, 2009 Copyright: 2009 Kim, Morrison. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This research was supported by Department of Microbiology, Immunology and Molecular Genetic